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點擊次數(shù):12578 發(fā)布時間:2020/12/29 15:32:04
【文獻標題】Effect of dehydroepiandrosterone on the immune response and gut microbiota in dextran sulfate sodium-induced colitis mice
【作者】Ji Cao,Huihui Zhang, Zhongmiao Yang,et.al
【作者單位】南京農(nóng)業(yè)大學(Nanjing Agricultural University)
【文獻中引用產(chǎn)品】
小鼠T細胞因子(TCF)elisa試劑盒
【關鍵詞】Dehydroepiandrosterone,Colitis,Immune function,Inflammatory,Gut microbiota
【DOI】https://doi.org/10.1016/j.molimm.2019.12.008
【影響因子(IF)】4.90
【出版期刊】《Molecular Immunology》
【產(chǎn)品原文引用】
Reagents
Dextran sulfate sodium (DSS, 36–50 kDa) was obtained from Meilun Biotech Co., Ltd. (Dalian, China). DHEA was obtained from Sigma (StLouis, MO, USA). 5-amino salicylic acid (5-ASA, a positive drug for UC therapy) was obtained from Yuanye Biotech Co., Ltd. (Shanghai,China). TRIZOL reagent kit, reverse transcriptase kit and SYBR Green Master Mix were obtainedfrom Vazyme Biotech Co., Ltd. (Nanjing,China). The MPO, MDA and SOD etection kits were obtained from Jiancheng Bioengineering Institute (Nanjing, China). The cytokine enzyme-linked immunosorbent assay (ELISA) kits were btained from Hengyuan Biotechnology Co., Ltd. (Shanghai, China). The Rabbit tip65, IkB-α, p38, phospho(p)-p65, p-IkB-α, p-p38 and iNOS were obtained from Sangon Biotechnology Co., Ltd. (Shanghai, China). The Rabbit anti-GAPDH, β-Tublin and HRP goat anti-rabbit IgG were obtained from Bioword Technology Co., Ltd. (Nanjing, China).
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